Franz Cell Permeability Assay (Synthetic Membrane)
In vitro permeability test using static Franz diffusion cells with Strat-M synthetic membrane for topical formulations. 6 samples at 1 time point (24 h). Reports steady-state flux J (µg/cm²/h) = amount permeated/(time × area) and apparent permeability Papp (cm/h) = (J × receptor volume)/(area × initial donor concentration), assuming linear permeation from single point.
Sample Requirement:
topical formulation (semi-solid or liquid) · 0.02 · grams
UV-VIS Anti-alpha-glucosidase Assay (pNPG substrate, 405 nm)
Spectrophotometric assay measuring inhibition of yeast alpha-glucosidase by sample extracts using p-nitrophenyl-α-D-glucopyranoside (pNPG) hydrolysis to p-nitrophenolate, detected at 405 nm. Alpha-glucosidase inhibitors inhibit the absorption of carbohydrates from the small intestine. They competitively inhibit enzymes that convert complex non absorbable carbohydrates into simple absorbable carbohydrates. These enzymes include glucoamylase, sucrase, maltase, and isomaltase.
Sample Requirement:
cosmetic product (cream, lotion, serum) · 5 · g
Sample Count:
Accepts exactly 1 sample.
Determination of total Antioxidant Activity using ABTS Assay
The antioxidant activity of the test sample was measured with the ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) assay by Microplate Reader which is the most popular method to evaluate the ability of compounds to scavenge free radicals. The ABTS assay was adapted to measure the antioxidant activity of natural products, extracts, and compounds.
Sample Count:
Accepts exactly 1 sample.
UV-VIS Total Flavonoids Content (Aluminum Chloride Colorimetric Assay)
UV-Vis spectrophotometric assay for total flavonoid content in plant extracts via complexation with aluminum chloride, quantified as quercetin equivalents at 420 nm.
Sample Requirement:
dry plant extract · 10 · mg
Sample Count:
Accepts exactly 1 sample.
UV-VIS Anti-Inflammation (Based-on Protein Denaturation) Activity Measurement
Egg albumin is employed as a model protein in the experiment, and denaturation is brought about by exposing it to extremes of heat, pH, or other denaturing agents. Egg albumin’s original conformation is disrupted during denaturation, changing its physical characteristics, and causing it to lose its functional activity. The egg albumin denaturation assay measures a drug or compound’s capacity to prevent or lessen egg albumin denaturation to evaluate its anti-inflammatory effects. The egg albumin denaturation assay is based on the idea that substances with anti-inflammatory qualities may be able to stabilize protein structures and prevent denaturation, which is frequently linked to inflammation and tissue damage. As a result, agents or chemicals that significantly decrease the denaturation o
UV-Vis Anti-Trypsin Inhibitory Assay
Colorimetric assay measuring inhibition of porcine trypsin hydrolysis of BAPNA substrate by absorbance at 410 nm. BAPNA is a manufactured protein substrate comprising of a color covalently bound to an amino acid. BAPNA has an amino acid with a dye molecule attached to it; enzymatic activity by trypsin breaks the bond between the amino acid and the dye molecule, coloring the clear solution yellow.
Sample Requirement:
Liquid extract or solution 2 mL
Sample Count:
Accepts exactly 1 sample.
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