Used in research to detect and quantify DNA double-strand breaks in cells. The kit measures phosphorylated histone H2AX (γH2AX), a biomarker that appears rapidly at sites of DNA damage. It is widely applied in studies of genotoxicity, cancer research, radiation biology, and drug development to assess the effectiveness of treatments that cause DNA damage. The ELISA format allows for high-throughput screening and provides a reliable, sensitive method to evaluate cellular responses to stress, toxins, or therapeutic agents. Commonly used in academic, pharmaceutical, and toxicological laboratories.