Isopropyl β-D-1-thiogalactopyranoside
98% biotech grade
- Product Code: 52205
Alias:
IPTG
CAS:
367-93-1
| Molecular Weight: | 238.3 g./mol | Molecular Formula: | C₉H₁₈O₅S |
|---|---|---|---|
| EC Number: | 206-703-0 | MDL Number: | MFCD00063273 |
| Melting Point: | 105 °C | Boiling Point: | |
| Density: | Storage Condition: | 2~8°C |
Product Description:
Isopropyl β-D-1-thiogalactopyranoside (IPTG) is widely used in molecular biology as a molecular mimic of allolactose, which induces the expression of genes under the control of the lac operon. It is particularly useful in the induction of protein expression in bacterial systems, such as E. coli, where it binds to the lac repressor, causing it to release from the operator region of the DNA. This allows RNA polymerase to transcribe the downstream genes, including those encoding for recombinant proteins. IPTG is favored over lactose for this purpose because it is not metabolized by the cells, ensuring a consistent level of induction. Additionally, it is used in blue-white screening to identify recombinant bacteria that have successfully incorporated a plasmid with an insert, as IPTG induces the production of β-galactosidase, which can cleave X-gal to produce a blue product in the absence of an insert. This makes IPTG an essential tool in cloning, protein production, and genetic engineering research.
Product Specification:
| Test | Specification |
|---|---|
| ASSAY | 98 100% |
| WATER BY KARL FISCHER | 0 2% |
| Melting point | 110 125? |
| pH 5 H2O | 5-7 |
| ABSORBANCE 5 WV SOLUBLE IN WATER | 0.06 AT 400NM |
| APPEARANCE | White crystalline powder |
| 14-DIOXANE | None detected |
Sizes / Availability / Pricing:
| Size (g) | Availability | Price | Quantity |
|---|---|---|---|
| 1.000 | 10-20 days | ฿500.00 |
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| 5.000 | 10-20 days | ฿1,360.00 |
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| 25.000 | 10-20 days | ฿5,160.00 |
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| 100.000 | 10-20 days | ฿17,980.00 |
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| 500.000 | 10-20 days | ฿59,800.00 |
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Isopropyl β-D-1-thiogalactopyranoside
Isopropyl β-D-1-thiogalactopyranoside (IPTG) is widely used in molecular biology as a molecular mimic of allolactose, which induces the expression of genes under the control of the lac operon. It is particularly useful in the induction of protein expression in bacterial systems, such as E. coli, where it binds to the lac repressor, causing it to release from the operator region of the DNA. This allows RNA polymerase to transcribe the downstream genes, including those encoding for recombinant proteins. IPTG is favored over lactose for this purpose because it is not metabolized by the cells, ensuring a consistent level of induction. Additionally, it is used in blue-white screening to identify recombinant bacteria that have successfully incorporated a plasmid with an insert, as IPTG induces the production of β-galactosidase, which can cleave X-gal to produce a blue product in the absence of an insert. This makes IPTG an essential tool in cloning, protein production, and genetic engineering research.
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