PAβN dihydrochloride is widely used as an efflux pump inhibitor in microbiological research. It is particularly effective in enhancing the activity of antibiotics against multidrug-resistant bacteria by blocking efflux pumps, which are mechanisms bacteria use to expel antibiotics. This application is crucial in studies aimed at overcoming antibiotic resistance and improving the efficacy of existing antimicrobial agents. Additionally, it is employed in laboratory settings to investigate bacterial efflux mechanisms and their role in drug resistance. Its use has also extended to screening potential therapeutic compounds that can work synergistically with antibiotics to combat resistant bacterial strains.