Prostaglandin F2β (tromethamine salt) is primarily used in veterinary medicine to induce luteolysis and estrus in livestock, particularly cattle and horses. It helps in synchronizing estrous cycles, making breeding programs more efficient. In human medicine, it is employed in ophthalmology to reduce intraocular pressure in conditions like glaucoma. Additionally, it is used in obstetrics to manage postpartum hemorrhage by promoting uterine contractions. Its ability to modulate smooth muscle activity makes it valuable in various therapeutic applications.