Used to measure 3β-hydroxysteroid dehydrogenase (3β-HSD) enzyme activity in mouse biological samples such as tissue lysates, cell extracts, or serum. This assay is commonly applied in endocrinology and reproductive biology research to study steroid hormone synthesis, particularly in adrenal glands, gonads, and placenta. It helps assess changes in enzyme activity under physiological or pathological conditions, including hormone-dependent diseases, developmental studies, and the impact of drugs or genetic modifications on steroidogenesis. The kit provides a quantitative, high-throughput method ideal for preclinical studies involving mouse models.