Used in biochemical and medical research to measure levels of 5-alpha reductase enzyme activity in rat tissue samples, particularly in studies related to steroid metabolism, hormonal disorders, and prostate health. Enables researchers to quantify enzyme expression in response to drugs, hormones, or disease states, supporting investigations into androgen-dependent conditions and potential therapeutic interventions. Commonly applied in preclinical studies to assess the efficacy of 5AR inhibitors and to understand endocrine pathways in rodent models.