Used in in vitro transcription applications where high yields of RNA are required with minimal double-stranded RNA (dsRNA) byproducts. This is particularly important for applications sensitive to innate immune responses, such as mRNA vaccine production, therapeutic RNA synthesis, and functional studies in mammalian cells, where dsRNA can trigger antiviral pathways. The low dsRNA formulation ensures cleaner RNA output, improving reproducibility and reducing off-target effects. It is also suitable for synthesizing RNA for microinjection, RNA interference (RNAi), CRISPR guide RNA preparation, and structural biology studies. The included 10X Transcription Buffer optimizes reaction conditions for consistent and efficient transcription.