Used in biomedical research to quantify levels of fatty acid β-oxidation enzymes in mouse tissue samples, particularly in liver and muscle. Helps assess mitochondrial function and energy metabolism in studies related to obesity, diabetes, and metabolic disorders. Enables researchers to evaluate how genetic modifications or drug treatments affect fatty acid metabolism in mouse models. Provides a reliable, high-throughput method for measuring enzyme activity indirectly through protein concentration, supporting mechanistic studies in metabolic disease research.