4-Methylumbelliferyl β-D-Glucuronide Hydrate
≥98%
Reagent
Code: #103725
CAS Number
881005-91-0
blur_circular Chemical Specifications
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Molecular Information
Weight
352.30(asAnhydrous) g/mol
Formula
C₁₆H₁₆O₉H₂O
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Registry Numbers
MDL Number
MFCD09039280
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Storage & Handling
Storage
room temperature
description Product Description
Used as a substrate in enzymatic assays to detect β-glucuronidase activity, which is crucial in various biological and medical research applications. It helps in identifying bacterial contamination in food and water by targeting E. coli, which produces β-glucuronidase. In clinical diagnostics, it aids in studying enzyme activity in tissues and cells, particularly in cancer research. Additionally, it is utilized in molecular biology for gene expression studies and as a fluorescent marker in histochemistry to visualize enzyme activity in cells and tissues. Its hydrolysis product, 4-methylumbelliferone, emits strong fluorescence under UV light, making it valuable for sensitive detection methods.
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Test Parameter | Specification |
---|---|
Appearance | White Solid |
Purity (%) | 97.5-100% |
Solubility | Colorless to very faint yellow, clear, 50 mg/mL pyridine |
Specific Rotation [α]20/D (C=0.2, H2O) | -125° to -110° |
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4-Methylumbelliferyl β-D-Glucuronide Hydrate
Used as a substrate in enzymatic assays to detect β-glucuronidase activity, which is crucial in various biological and medical research applications. It helps in identifying bacterial contamination in food and water by targeting E. coli, which produces β-glucuronidase. In clinical diagnostics, it aids in studying enzyme activity in tissues and cells, particularly in cancer research. Additionally, it is utilized in molecular biology for gene expression studies and as a fluorescent marker in histochemistry to visualize enzyme activity in cells and tissues. Its hydrolysis product, 4-methylumbelliferone, emits strong fluorescence under UV light, making it valuable for sensitive detection methods.
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