Galβ(1-3)GlcNAc-β-pNP is widely used in biochemical and enzymatic research as a substrate to study glycosidase and glycosyltransferase activities. It is particularly valuable for investigating the specificity and kinetics of enzymes involved in carbohydrate metabolism. This compound is also employed in the development of assays to detect and measure enzyme activity in various biological samples. Additionally, it serves as a tool in glycobiology research to understand the structure and function of glycans and their role in cellular processes. Its application extends to the screening of enzyme inhibitors, which can be potential therapeutic agents for diseases related to glycosylation disorders.