Used as a substrate in enzymatic assays to study the activity of glycosidases, particularly those that cleave β-linked glycosidic bonds. Upon reaction with the enzyme, it releases p-nitrophenol (pNP), which can be measured spectrophotometrically at 400-410 nm, allowing precise assessment of enzyme activity. It is valuable in biochemical research for understanding enzyme mechanisms and kinetics. Applied in the development of diagnostic tools for detecting enzyme deficiencies or abnormalities. Also utilized in the synthesis of glycoconjugates and in the investigation of carbohydrate metabolism pathways. Its application extends to the screening of potential inhibitors for therapeutic purposes, including diseases related to enzyme malfunctions such as glycosphingolipid storage diseases or diabetes.