Used in immunological research to quantify RORγt protein levels in cell lysates or tissue samples. Essential for studying Th17 cell differentiation and function, which play key roles in autoimmune diseases, inflammation, and host defense. Enables researchers to assess the impact of drugs, cytokines, or genetic modifications on RORγt expression. Widely applied in preclinical studies related to multiple sclerosis, psoriasis, inflammatory bowel disease, and cancer immunotherapy. Provides a reliable, high-throughput method for monitoring transcriptional regulation in immune cells.