Used in biomedical research to quantify levels of human α-enolase in biological samples such as serum, plasma, and cell lysates. This ELISA kit enables detection and measurement of α-enolase, a glycolytic enzyme that also functions as a plasminogen receptor on cell surfaces. It is particularly useful in studies related to cancer, where α-enolase is often overexpressed on tumor cells and contributes to tissue invasion and metastasis. The assay supports research into autoimmune diseases, as α-enolase can act as an autoantigen in conditions like systemic lupus erythematosus and antiphospholipid syndrome. It is also employed in infectious disease research, as certain pathogens bind to surface-expressed α-enolase to facilitate host cell entry. The kit provides a sensitive and specific tool for monitoring disease progression and evaluating therapeutic responses in experimental models.