Used in biological research to quantify β-actin protein levels in rat tissue or cell lysates. Commonly applied in studies involving protein expression, cellular structure, and as a loading control in experiments assessing changes in total protein content. Helps normalize data in assays evaluating cytoskeletal dynamics, muscle contraction, cell motility, and intracellular transport. Widely utilized in Western blotting validation, gene knockdown studies, and drug effect analysis where consistent protein loading is critical. Suitable for high-throughput screening and reproducible quantification in experimental and control samples.