Used to measure levels of tumor necrosis factor-alpha (TNF-α) in human biological samples such as serum, plasma, or cell culture supernatants. It is widely applied in research and clinical settings to study inflammatory diseases including rheumatoid arthritis, Crohn's disease, and sepsis. The kit helps monitor immune responses, evaluate drug efficacy in anti-inflammatory therapies, and support biomarker discovery in immunology and oncology research. Its high sensitivity and specificity make it suitable for detecting low concentrations of TNF-α in small sample volumes.