Used in biomedical research to quantify levels of active interleukin-1β in cell cultures, tissue extracts, and biological fluids such as serum or cerebrospinal fluid. Helps investigate inflammatory processes in diseases like rheumatoid arthritis, Alzheimer's, and sepsis. Enables researchers to evaluate the effectiveness of anti-inflammatory compounds by measuring changes in IL-1β concentration. Commonly applied in drug development, immunology studies, and clinical diagnostics research.