Used to measure levels of tumor necrosis factor-alpha (TNF-α) in biological samples from mice, such as serum, plasma, or cell culture supernatants. Commonly applied in research on inflammation, autoimmune diseases, and immune responses. Helps evaluate the effectiveness of anti-inflammatory drugs and study disease mechanisms in mouse models of conditions like arthritis, sepsis, and neurodegenerative disorders. Provides a sensitive and specific way to quantify TNF-α protein, enabling accurate assessment of immune system activity.