Used to detect and quantify mouse tumor necrosis factor-alpha (TNF-α) in biological samples such as serum, plasma, cell culture supernatants, and tissue extracts. Due to its high sensitivity, it is ideal for measuring low levels of TNF-α, which is a key cytokine involved in systemic inflammation, immune response, and various disease models including arthritis, sepsis, and autoimmune disorders. Commonly used in preclinical research to evaluate inflammatory responses in mouse models, assess drug efficacy, and study signaling pathways related to inflammation and cell death.