Used to measure soluble RANK Ligand levels in biological samples such as serum, plasma, or cell culture supernatants from mice. This assay is widely applied in bone metabolism research, particularly in studies related to osteoclast formation, bone resorption, and diseases like osteoporosis, arthritis, and periodontal disease. It helps evaluate the role of the RANK/RANKL/OPG pathway in immune and skeletal systems, making it valuable in inflammation and autoimmune disease research. The kit enables quantitative analysis, supporting both preclinical studies and drug development targeting bone loss or immune-mediated bone disorders.