Used to measure rat interferon-gamma (IFN-γ) levels in biological samples such as serum, plasma, or cell culture supernatants. Commonly applied in immunology research to study immune responses, inflammation, and T-cell activity in rat models. Helps evaluate the effectiveness of drugs or vaccines by monitoring cytokine production. Widely used in preclinical studies involving infectious diseases, autoimmunity, and cancer immunotherapy. Provides a sensitive and specific way to quantify IFN-γ without radioactivity or complex instrumentation.