Used to measure levels of tumor necrosis factor-alpha (TNF-α) in biological samples from rats, such as serum, plasma, or cell culture supernatants. Widely applied in research on inflammation, autoimmune diseases, and immune responses. Helps evaluate the effectiveness of anti-inflammatory drugs and study disease mechanisms in rat models of arthritis, sepsis, and other inflammatory conditions. Provides a sensitive and specific way to quantify TNF-α, enabling accurate assessment of immune system activity.