β-Glycerol phosphate disodium salt hydrate is widely used in biochemical and cell culture applications. It serves as a substrate for alkaline phosphatase assays, helping to measure enzyme activity in various biological samples. In cell culture, it is utilized as a source of phosphate ions, which are essential for cellular metabolism and energy production. Additionally, it is employed in studies related to bone mineralization and osteoblast differentiation, as it plays a role in promoting the formation of hydroxyapatite, a key component of bone tissue. Its stability and solubility make it a preferred choice in research involving phosphate metabolism and signaling pathways.