This compound is widely used in peptide synthesis, particularly in solid-phase peptide synthesis (SPPS) methods. It serves as a protected form of threonine, where the Fmoc (9-fluorenylmethoxycarbonyl) group protects the amino group, and the tert-butyl ester protects the carboxyl group. This protection ensures selective reactions during peptide chain assembly, preventing unwanted side reactions. It is especially valuable in synthesizing complex peptides and proteins for research in biochemistry, pharmaceuticals, and biotechnology. Additionally, its stability and ease of deprotection make it a preferred choice for producing peptides with high purity and yield.