Used in biomedical research to quantify levels of IKBα protein in cell lysates or tissue extracts. Helps study the regulation of the NF-κB signaling pathway, which plays a key role in inflammation, immune response, cell survival, and cancer development. Enables researchers to assess how stimuli such as cytokines, pathogens, or drugs affect IKBα degradation and subsequent NF-κB activation. Commonly applied in pharmacological studies evaluating anti-inflammatory compounds or signaling mechanisms in diseases like arthritis, sepsis, and various cancers. Provides a reliable, high-throughput method for monitoring intracellular protein changes in experimental models.