Used in biochemical and medical research to measure levels of carnitine palmitoyltransferase 1α (CPT1α) in mouse samples. This enzyme plays a key role in fatty acid metabolism, particularly in the regulation of long-chain fatty acid transport into mitochondria for β-oxidation. The ELISA kit enables researchers to quantify CPT1α protein expression in tissues such as liver, muscle, and adipose tissue, supporting studies on metabolic disorders including obesity, diabetes, and non-alcoholic fatty liver disease (NAFLD). It is also applied in pharmacological research to evaluate the effects of drugs or genetic modifications on mitochondrial metabolism and energy homeostasis in mouse models. The assay is valued for its sensitivity, specificity, and ability to process multiple samples simultaneously, making it suitable for high-throughput screening in experimental studies.