Used in biomedical research to quantitatively measure levels of human α-enolase (ENO1) in biological samples such as serum, plasma, cell lysates, and tissue homogenates. This ELISA kit is particularly valuable in studies related to cancer, where ENO1 is overexpressed in various tumors including lung, breast, and pancreatic cancers, serving as a potential biomarker for tumor progression and diagnosis. It is also applied in autoimmune disease research, as α-enolase has been identified as a target of autoantibodies in conditions like rheumatoid arthritis and systemic lupus erythematosus. Additionally, the kit supports neuroscience research, given ENO1's role in neuronal protection and its implication in neurodegenerative disorders. The assay enables high-throughput, sensitive, and specific detection, making it essential for both clinical research and drug development.