Used in biomedical research to measure hypoxia inducible factor 1β (HIF-1β) levels in rat samples. Helps investigate cellular responses to low oxygen conditions. Commonly applied in studies of cancer, cardiovascular diseases, and metabolic disorders. Enables researchers to evaluate HIF-1β expression under experimental treatments, aiding in drug development and mechanistic studies. Provides a quantitative and sensitive method for protein detection in tissue homogenates and cell lysates.