Used in neuroscience and clinical research to quantify S100β protein levels in biological samples such as serum, plasma, cerebrospinal fluid, and tissue homogenates. The kit helps assess blood-brain barrier integrity and glial cell activity, making it valuable in studies related to traumatic brain injury, stroke, neurodegenerative diseases like Alzheimer's, and brain tumors. It is also applied in monitoring neonatal hypoxic-ischemic encephalopathy and evaluating central nervous system damage in sports medicine and critical care. The ELISA format allows for high sensitivity and specificity, enabling reliable detection of S100β concentration changes in experimental and diagnostic settings.