This compound is primarily used in biomedical research as an internal standard in mass spectrometry-based assays. It helps in the accurate quantification of androstanediol glucuronide in biological samples, such as urine or blood, by correcting for variations in sample preparation and instrument performance. Its deuterated form ensures it can be distinguished from the endogenous analyte, providing reliable and precise measurements. This application is particularly valuable in studies related to steroid metabolism, endocrine disorders, and the development of diagnostic tools for hormonal imbalances.