This compound is primarily utilized in the development of immunoassays for the detection and quantification of hormones. It serves as a key component in the preparation of immunogens, which are used to generate antibodies specific to estradiol. These antibodies are essential in various diagnostic kits, enabling the measurement of estradiol levels in biological samples such as blood or urine. The compound’s structure allows it to mimic estradiol, making it highly effective in competitive binding assays. Additionally, it is employed in research studies to investigate hormone-related physiological processes and disorders, contributing to advancements in endocrinology and reproductive health.