Used in the synthesis of bile acid derivatives and plays a role in the study of liver metabolism and cholesterol breakdown. It serves as an intermediate in the preparation of pharmaceuticals targeting digestive disorders and metabolic diseases. Also utilized in research to understand bile acid signaling pathways, which are important in regulating cholesterol homeostasis, glucose metabolism, and inflammation. Its structure makes it valuable in developing diagnostic agents and enzyme inhibitors related to steroid and lipid metabolism. Additionally, it can serve as a standard in chromatographic analysis for biomolecules related to bile acids.