Used in molecular biology for precise DNA cleavage at specific recognition sites, enabling gene mapping and restriction analysis. Commonly applied in cloning workflows to verify plasmid constructs and in genotyping studies. Its high specificity and fast digestion kinetics make it suitable for high-throughput applications where rapid processing is required. Also utilized in diagnostic assays to detect genetic variations by generating distinct fragment patterns. Stable under standard reaction conditions, it supports streamlined protocols without the need for extended incubation times.