Used in biomedical research to quantify hypoxia-inducible factor alpha (HIF-α) levels in rat tissue or cell samples. This ELISA kit enables researchers to study cellular responses to low oxygen conditions, commonly involved in processes like tumor development, ischemia, and inflammation. It is widely applied in preclinical studies to evaluate changes in HIF-α expression under various experimental treatments, helping to understand signaling pathways and assess potential therapeutic interventions. The assay provides a sensitive and specific measurement, supporting research in oncology, cardiovascular diseases, and metabolic disorders.