Used in biomedical research to quantify β-hexosaminidase enzyme activity in rat samples, this ELISA kit is particularly valuable in studies related to inflammation, mast cell activation, and allergic responses. The assay enables researchers to measure degranulation of mast cells, as β-hexosaminidase is released during this process, providing a reliable marker for immune cell activity. It is widely applied in immunology and pharmacology research to evaluate the effects of drugs, toxins, or experimental compounds on mast cell stability and allergic pathways in rat models. The kit offers high sensitivity and specificity, allowing for accurate detection in cell culture supernatants and tissue homogenates.