Used in biomedical research to quantify total hypoxia-inducible factor 1-alpha (HIF-1α) levels in mouse tissue lysates or cell extracts. This assay helps researchers study cellular responses to low oxygen conditions, particularly in processes such as tumor development, angiogenesis, metabolic adaptation, and inflammation. It is widely applied in cancer research, cardiovascular disease studies, and drug development where HIF-1α signaling pathways are investigated. The kit enables sensitive and specific detection, allowing for high-throughput analysis in experimental models involving mice.