Used in research settings to quantitatively measure 16α-hydroxyestrone (16α-OHE1) levels in human biological samples such as serum, plasma, or urine. This assay is commonly applied in studies related to estrogen metabolism, hormonal balance, and conditions linked to estrogenic activity including breast cancer, endometriosis, and metabolic disorders. The kit supports investigation into the ratio of 16α-OHE1 to other estrogen metabolites, which is considered a potential biomarker for assessing cancer risk and evaluating the impact of dietary or therapeutic interventions on hormone metabolism. It is not intended for diagnostic use but serves as a tool for academic, clinical, and pharmaceutical research.