L-Homopropargyl glycine is primarily used in biochemical research as a tool for studying protein synthesis and post-translational modifications. It serves as an analog for the amino acid methionine, allowing researchers to selectively label proteins in vivo. This labeling is particularly useful for tracking protein dynamics, interactions, and localization within cells. Additionally, its alkyne functional group enables bioorthogonal reactions, such as click chemistry, facilitating the attachment of fluorescent dyes or other probes for visualization and analysis. This makes it a valuable compound in proteomics, cell biology, and drug discovery studies.