Calpain inhibitor II is widely used in biochemical research to study the role of calpain enzymes in various cellular processes. It is particularly valuable in investigating conditions such as neurodegenerative diseases, muscle disorders, and ischemia-reperfusion injury, where calpain activity is implicated. By inhibiting calpain, researchers can better understand its function in cell signaling, apoptosis, and cytoskeletal remodeling. Additionally, it is utilized in experimental models to explore potential therapeutic strategies for diseases associated with excessive calpain activation. Its application extends to preserving cellular integrity in studies involving tissue damage or stress, making it a crucial tool in both basic and applied biomedical research.