Nuclear fast red is widely used in histology and pathology as a staining agent. It is particularly effective in highlighting calcium deposits in tissues, making it valuable for diagnosing conditions like calcification in arteries or kidney stones. The dye is often employed in conjunction with other stains, such as Alcian blue, to differentiate between various tissue components. Additionally, it is used in bone histology to visualize osteoid seams and mineralized bone, aiding in the study of bone formation and remodeling. Its ability to bind selectively to specific tissue structures makes it a crucial tool in both research and clinical diagnostics.