2-Fluoroadenine is primarily used in biochemical and medical research due to its ability to inhibit DNA and RNA synthesis. It is often employed as an antimetabolite in studies focusing on cell proliferation and cancer research, as it can interfere with the growth of certain types of cells. Additionally, it serves as a valuable tool in understanding nucleic acid metabolism and the mechanisms of enzyme inhibition. Its application extends to the development of antiviral and anticancer agents, where it helps in identifying potential therapeutic targets.