Iodonitrotetrazolium chloride (INT)

98%

  • Product Code: 123023
  Alias:    Iodonitrotetrazolium violet (INT); Iodonitrotetrazolium blue chloride, 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazolium chloride hydrate, 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride chloride hydrate
  CAS:    146-68-9
Molecular Weight: 505.71 g./mol Molecular Formula: C₁₉H₁₃N₅O₂ICl
EC Number: MDL Number: MFCD00011961
Melting Point: 240 °C (dec.)(lit.) Boiling Point:
Density: Storage Condition: 2~8°C, away from light
Product Description: Iodonitrotetrazolium chloride (INT) is widely used as a redox indicator in biochemical and microbiological assays. It is particularly useful in the detection of dehydrogenase activity, where it changes from colorless to a red formazan product upon reduction, making it a valuable tool for visualizing metabolic activity in cells. In microbiology, INT is employed to assess bacterial respiration and viability, as it is reduced by active electron transport chains in living cells. Additionally, it is utilized in histochemistry to localize enzyme activity in tissue samples, providing insights into cellular function and health. Its application in cytotoxicity assays also helps in evaluating the effects of drugs or toxins on cell viability.
Product Specification:
Test Specification
Water By Karl Fischer 0-6
Purity (Titration With Agno3) 97.5-102.5
Appearance White To Dark Yellow To Dark Orange Powder Or Crystals
Infrared Spectrum Conforms To Structure
Melting Point 231 Degree Celsius (dec.)
Sizes / Availability / Pricing:
Size (g) Availability Price Quantity
0.500 10-20 days $65.62
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1.000 10-20 days $102.99
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5.000 10-20 days $401.17
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25.000 10-20 days $1,951.86
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Iodonitrotetrazolium chloride (INT)
Iodonitrotetrazolium chloride (INT) is widely used as a redox indicator in biochemical and microbiological assays. It is particularly useful in the detection of dehydrogenase activity, where it changes from colorless to a red formazan product upon reduction, making it a valuable tool for visualizing metabolic activity in cells. In microbiology, INT is employed to assess bacterial respiration and viability, as it is reduced by active electron transport chains in living cells. Additionally, it is utilized in histochemistry to localize enzyme activity in tissue samples, providing insights into cellular function and health. Its application in cytotoxicity assays also helps in evaluating the effects of drugs or toxins on cell viability.
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