Used in research settings as an intermediate in steroid metabolism studies, particularly in examining cholesterol oxidation pathways and the formation of oxysterols. It serves as a reference compound in analytical methods for detecting steroidal byproducts in biological samples. Also utilized in the development and calibration of assays related to lipid peroxidation, which is linked to oxidative stress and various degenerative diseases. Its structural properties make it valuable in studying membrane dynamics and sterol-protein interactions in cellular models.