Alkaline phosphatase
≥10 DEA units/mg, lyophilized powder, derived from bovine intestinal mucosa
- Product Code: 98968
Alias:
alkaline phosphatase
CAS:
9001-78-9
Properties:
Soluble in water and dilute buffer, pI5.7, optimum pH 8.0-10.5 (depending on the substrate)
| Molecular Weight: | Molecular Formula: | NULL | |
|---|---|---|---|
| EC Number: | 232-631-4 | MDL Number: | MFCD00131849 |
| Melting Point: | Boiling Point: | 1.41 °C | |
| Density: | 1.12 g/mL at 20 °C | Storage Condition: | -20°C, dry, sealed |
Product Description:
Alkaline phosphatase is widely used in molecular biology and biochemistry for various applications. It is commonly employed in the dephosphorylation of DNA, RNA, and proteins, which is essential for preventing self-ligation in cloning experiments. This enzyme is also utilized in ELISA and other immunoassays as a reporter molecule, where it catalyzes colorimetric or chemiluminescent reactions to detect target molecules. In clinical diagnostics, alkaline phosphatase serves as a biomarker for liver and bone disorders, with elevated levels often indicating conditions such as hepatitis, cirrhosis, or bone diseases. Additionally, it is used in histochemistry to visualize tissue-specific enzyme activity, aiding in the study of cellular processes and disease pathology. Its versatility and stability make it a valuable tool in both research and medical applications.
Product Specification:
| Test | Specification |
|---|---|
| APPEARANCE | Powder |
| Infrared spectrum | Conforms to Structure |
Sizes / Availability / Pricing:
| Size (g) | Availability | Price | Quantity |
|---|---|---|---|
| 0.100 | 10-20 days | ฿8,200.00 |
+
-
|
| 0.500 | 10-20 days | ฿31,000.00 |
+
-
|
Alkaline phosphatase
Alkaline phosphatase is widely used in molecular biology and biochemistry for various applications. It is commonly employed in the dephosphorylation of DNA, RNA, and proteins, which is essential for preventing self-ligation in cloning experiments. This enzyme is also utilized in ELISA and other immunoassays as a reporter molecule, where it catalyzes colorimetric or chemiluminescent reactions to detect target molecules. In clinical diagnostics, alkaline phosphatase serves as a biomarker for liver and bone disorders, with elevated levels often indicating conditions such as hepatitis, cirrhosis, or bone diseases. Additionally, it is used in histochemistry to visualize tissue-specific enzyme activity, aiding in the study of cellular processes and disease pathology. Its versatility and stability make it a valuable tool in both research and medical applications.
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