Used in biomedical research to quantitatively measure β-catenin protein levels in cell lysates, tissue extracts, or biological fluids. This ELISA kit is particularly useful in studying Wnt signaling pathway activity, which plays a critical role in embryonic development, cell proliferation, and cancer progression. Researchers use it to assess changes in β-catenin expression under various experimental conditions, including drug treatments, gene knockdowns, or disease states like colorectal cancer and other malignancies. The assay provides high sensitivity and specificity, enabling accurate detection of intracellular β-catenin, helping to validate signaling pathway modulation in both basic research and drug development.