5α-reductase-IN-1 is primarily used in research settings to study the inhibition of the 5α-reductase enzyme, which plays a critical role in the metabolism of steroid hormones. By blocking this enzyme, it helps in understanding the pathways involved in conditions like benign prostatic hyperplasia (BPH) and androgenetic alopecia. Researchers utilize this compound to explore potential therapeutic strategies for managing these conditions. Additionally, it serves as a valuable tool in biochemical assays to evaluate the efficacy of other 5α-reductase inhibitors, aiding in the development of new drugs targeting hormone-related disorders.