4-Nitroacetanilide is primarily used as an intermediate in the synthesis of various dyes and pigments. It plays a crucial role in the production of azo dyes, which are widely used in the textile industry for coloring fabrics. Additionally, it serves as a precursor in the manufacture of pharmaceuticals, particularly in the synthesis of certain analgesics and antipyretics. In organic chemistry, it is utilized as a reagent for nitration reactions, helping to introduce nitro groups into other compounds. Its applications also extend to research and development, where it is used in studies involving chemical reactions and molecular transformations.