Widely used in dietary supplements and fortified foods to prevent folate deficiency, supporting red blood cell formation and reducing the risk of anemia. Essential during pregnancy to prevent neural tube defects in developing fetuses, making it a key component in prenatal vitamins. Used in combination with other B vitamins to support cardiovascular health by helping regulate homocysteine levels. Also applied in cell culture media for growing mammalian cells, due to its role in DNA synthesis and repair. Commonly added to cereals, grains, and flour in public health programs to improve population-wide folate status.