Widely used in metal ion chelation, particularly in biochemical and analytical applications. Its structure allows effective binding of divalent metal ions such as calcium, magnesium, and transition metals, making it useful in buffer systems where metal ion control is critical. Commonly employed in enzymatic assays to inhibit metalloenzymes or to stabilize enzyme activity by removing trace metal interferences. Also utilized in the preparation of metal-depleted solutions in cell culture and protein purification workflows. Its derivative forms serve in the development of metal ion indicators and sensors due to its stable complexation properties.