DNA-Grade EDTA Solution
0.5M,pH8.0
- Product Code: 96737
CAS:
60-00-4
| Molecular Weight: | 292.24 g./mol | Molecular Formula: | C₁₀H₁₆N₂O₈ |
|---|---|---|---|
| EC Number: | 200-449-4 | MDL Number: | MFCD00003541 |
| Melting Point: | Boiling Point: | ||
| Density: | Storage Condition: | 2-8°C |
Product Description:
DNA-grade EDTA solution is widely used in molecular biology and genetic research for its ability to chelate metal ions, particularly magnesium and calcium. This property makes it essential for stabilizing DNA and RNA by inhibiting nucleases that require these metal ions for activity. It is a key component in DNA extraction and purification protocols, ensuring the integrity of nucleic acids during isolation. Additionally, it is used in PCR and other enzymatic reactions to prevent metal-dependent degradation. In cell culture, it aids in detaching adherent cells by disrupting calcium-dependent cell adhesion proteins. Its role in preserving sample quality and enhancing experimental accuracy makes it indispensable in various laboratory applications.
Product Specification:
| Test | Specification |
|---|---|
| APPEARANCE | liquid |
| CONCENTRATION | 1.0molL |
| PH | pH8.0 |
| Infrared spectrum | Conforms to Structure |
Sizes / Availability / Pricing:
| Size (g) | Availability | Price | Quantity |
|---|---|---|---|
| 100.000 | 10-20 days | $57.42 |
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| 250.000 | 10-20 days | $117.19 |
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| 500.000 | 10-20 days | $204.50 |
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DNA-Grade EDTA Solution
DNA-grade EDTA solution is widely used in molecular biology and genetic research for its ability to chelate metal ions, particularly magnesium and calcium. This property makes it essential for stabilizing DNA and RNA by inhibiting nucleases that require these metal ions for activity. It is a key component in DNA extraction and purification protocols, ensuring the integrity of nucleic acids during isolation. Additionally, it is used in PCR and other enzymatic reactions to prevent metal-dependent degradation. In cell culture, it aids in detaching adherent cells by disrupting calcium-dependent cell adhesion proteins. Its role in preserving sample quality and enhancing experimental accuracy makes it indispensable in various laboratory applications.
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